Persönlicher Status und Werkzeuge



New papers from van der Linde lab.

Application of the pathogen Trojan horse approach in maize (Zea mays).

Plant Signaling and Behavior

Karina van der Linde, Rachel L. Egger, Ljudmilla Timofejeva, Virginia Walbot (2018).

Maize, Zea mays, the second-most-widely-grown crop, yields 20 % of all consumed calories worldwide.1Despite its agronomic importance, research progress is limited by costly transformation. We recently described the Trojan horse method as a useful tool to study maize proteins in situ that circumvents time- and space-consuming whole plant transformation. The Trojan horse approach uses the protein-folding and secretory properties of the corn smut fungus Ustilago maydis to secrete maize proteins from fungal cells into the maize apoplast. Here, we discuss the timing and location of U. maydis during infection and the protein secretion site in relation to anther anatomy. This spatiotemporal analysis enables the study of apoplastic anther proteins in various premeiotic anther developmental stages, and could be adapted for larger screens.



Chapter Ten - Pre-meiotic anther development.

Curr. Top. Dev. Biol.

Karina van der Linde and Virginia Walbot (2019). 

Most genetic and molecular analyses of anther development utilize Arabidopsis thalianaOryza sativa (rice), and Zea mays (maize). Especially in maize, early stages of anther development are easy to study because: (1) Maize has unisex flowers. (2) Compared to rice or A. thaliana, maize anthers are relatively large, making dissection for molecular and biochemical analyses easy. (3) Anther developmental stage is strongly correlated with maize anther length. Besides these technical advantages, understanding anther and pollen development in maize is of significant agricultural importance. Today maize is a worldwide cereal crop: approximately 25% of all consumed food contains maize. Yield stability or even increases depend on maintenance of hybrid vigor, and production of hybrid seed requires manual detasseling or genetic control of pollen development. Knowledge of pollen development can also be used to manage transgene containment.

In the first section of this chapter, we will describe the current model for sequential cell fatespecification in maize anther lobes, with reference to rice and A. thaliana to point out similarities and differences. In the second section of this chapter, we will review what is known about the individual cell types in anther lobes. The diversity of anther organization is addressed to a limited extent by cytological studies of anthers, often directed to clarify taxonomic relationships. In the third section, we will comment on how new lines of investigation could clarify questions remaining in our current appreciation of anther development.